Oxidative Stress Probe
Probe in healthy cells
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Long term collaboration between the Robert Gordon University and The Rowett Research Institute led to the development of a novel lipid soluble flavonoid that has application as a therapeutic antioxidant. It was also later discovered that in its natural reduced state, the flavonoid is highly fluorescent. The technology available relates to the use of the fluorescent antioxidant as a tool to measure real time oxidative stress.
Oxidation has toxic effects through the production of peroxides and free radicals that damage all components of the cell, including proteins, lipids, and DNA. Oxidative stress contributes to human diseases such as atherosclerosis, Parkinson's disease, Heart Failure, Myocardial Infarction and Alzheimer's disease. High sensitivity, flexibility and ease of analysis have ensured that fluorescent probes are an established and rapidly expanding area in biological assay development.Key Benefits
- Our flavonoid compound is rapidly distributed within cells and appears preferentially concentrated within mitochondria which may have disease specific application.
- Oxidative stress induces a stress-dose related decrease in the level of fluorescence.
- This change in fluorescence has been successfully monitored by fluorescence/confocal microscopy but may be measured on alternate fluorimetric platforms such as conventional fluorimetry and flow cytometry.
- The real time activity allows qualitative and quantitative measurement of the amount and rate of oxidation without adversely affecting the cell.
Applications
- Our fluorescent lipid soluble flavonoid has many applications in assessing the role of oxidative injury
- Comparison of the impact of oxygen ions, free radicals, and peroxides on different types of cells to gauge the efficacy of different therapies or the impact of oxidising environmental factors
- real time measurement of metabolic processes
- real time prevention and measurement of oxidation in foods, specifically those containing unsaturated fats
- real time prevention and measurement of oxidation in fuels, lubricants and polymers such as rubbers, plastics and adhesives
IP Status
Patent Pending
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